Reporter

Part:BBa_K4245136:Experience

Designed by: Bria S VarnBuhler, Jared Moon, Sourav Kumar Dey, Jiahui Wu, Samie R Jaffrey, Akshaya Poonepalle, Shivaek Venkateswaran, Varnica Basavaraj, Manaswi Gorle, Sahana Ram Narayanan, Daeun Lee, Janet Standev   Group: iGEM22_Lambert_GA   (2022-10-09)


The Lettuce split aptamer has been tested with RCP produced through Rolling Circle Amplification (RCA). RCA was run with hsa-miR-1-3p RCA Padlock Probe (BBa_K4245200). The products, split Lettuce, and DFHBI-1T were mixed and heated at 70°C for 5 minutes, then cooled and held at 41°C for 1 hour. The fluorescence before and after were measured on the plate reader. (see Fig. 1)


Figure 1. Graph of split Lettuce reaction with RCP. The values represent the change in fluorescence before and after the reaction with DFHBI-1T took place.


The increase in fluorescence of the RCP + Lettuce + dye was not significantly greater than the controls, which suggests that the split Lettuce likely did not successfully bind the RCP and did not induce fluorescence in DFHBI-1T.


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